Supplementation of Activated Carbon for the Increased Production of Acid α-glucosidase in Rice Cell Suspension Cultures

초록

Pompe disease is caused by the deficiency of lysosomal acid α-glucosidase (GAA) enzyme. GAA could be produced in transgenic rice cells using RAmy3D promoter which expresses target proteins by sugar depletion. However, rice cells sometimes display browning which results in a decreased viability, when the cells are inoculated to sugar-free medium for GAA production. Therefore, browning has to be prevented for increasing the production of target proteins in plant cell cultures [1]. Activated carbon (AC) is known to show positive effects on plant cultures. AC can reduce the oxidation of phenolic compounds, which prevent the cell death caused by browning. Also, AC can act as an adsorbent for various toxic substances. In this study, in order to characterize the positive effects of AC on GAA productivity in rice cell suspension cultures, three different concentrations of AC (0.025%, 0.05%, and 0.1%) were supplemented in culture medium compared to that of control. It was found that the addition of 0.025% AC exhibited the highest GAA productivity without lowering viability. This result means that the addition of AC could enhance the production of target proteins in rice cell cultures.