Effects of stabilizing agents on hGM-CSF production in suspension cell cultures of Oryza sativa L.

초록

Human granulocyte-macrophage stimulating factor (hGM-CSF) is a naturally occurring cytokine that stimulates the production and antibacterial function of neutrophils and monocytes. Lately, thanks to the developments of genetic engineering, hGM-CSF can be produced by suspension cultures of genetically modified rice cells. However, the secreted hGM-CSF is unstable in the medium. In order to decrease the loss of the protein, two kinds of stabilizing agents were added in the medium. Polyvinylpyrrolidone (PVP) and gelatin well known as widespread stabilizers were used in this experiment. Gelatin was more effective in stabilizing the secreted hGM-CSF than PVP. Then, to decide the optimum concentration of gelatin, 0.2, 1, 2 and 3%(w/v) gelatin were added in the medium. The addition of 0.2% gelatin showed the best result. The maximum hGM-CSF concentration reached 28.4 mg/L, a 2.14-fold increase compared to that of control.