Osteoporosis biomarker 'c-terminal telopeptide' identification on carbon nanofiber-modified interdigitated electrode sensor

초록

Osteoporosis is a bone disease, characterized with bone tissue deterioration and decreasing level of bone mineral. Appropriate diagnostic is mandatory to prevent osteoporosis and provide a space for enough surgical and pharmacologic treatments. Available radiological techniques cause various health issues and research proved that biological markers are playing a major role for bone mineralization, can be a good alternative for diagnosing osteoporosis. C-terminal telopeptide (CTx) has been found as a potential biomarker, this study uses interdigitated electrode (IDE) sensor to identify CTx. Carbon nanofiber (CNF) measured as 50 nm by high-resolution microscope was used to modify the sensing electrode surface through amine-linker and it promotes higher anti-CTx antibody immobilization. CTx was quantified on this antibody anchored surface and attained the limit of detection to be 25 pg/mL. A regression co-efficient [y = 0.566x - 0.2627, R-2 = 0.9823] with triplicates (n = 3) determined the high-performance of IDE. Control molecules failed to increase the current flow, is indicating the selective CTx detection and CTx serum spiked human serum provided an increment in current-volt at linear concentrations are from 25 to 800 pg/mL, indicating the selective detection.

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