Evaluation of a Electrospun nano-fibrous nonvascular drug eluting stent

초록

OBJECTIVES: The recent introduction of drug-eluting stents (DES) has been suggesting a potential way to treat or relieve restenosis. The metallic stent being able to elute paclitaxel (PTX) from electrospun poly(urethane) (PU) was prepared for the application toward stent-based drug delivery and local treatment of malignant tumors. The electrospun PU-PTX nano-fibers could allow homogeneous drug loading and sustained release of PTX up to 30 days. We evaluated in vitro and in vivo performance of PTX-loaded stents with respect to morphology, PTX release, and animal study to observe suppression of tumors. Annexin V binding study and histology (TUNEL assay and H&E staining) had also confirmed the long term biological activity of the released PTX and the apoptosis of tumor tissues after the localization of PTX. METHODS: PTX eluting PU nano-fibers was electrospun on bare metallic stent with 0.5ml/hr flow rate for 4hrs of 0.7kV conditions. We progressed listed processes for evaluate in vitro and in vivo performance of electrospun PU nano-fibrous DES; morphology, release test, animal study to observe suppression of tumor growth. Compared four groups; nontreat, electrospun PU nano-fibrous membrane (ESM), PTX-injection (PTX-inj) and electro-spun PU-PTX nano-fibrous membrane (PTX-ESM). RESULTS: The release of PTX from electrospun PU nano-fibrous DES was sustained for 30days (Fig. 1). Subcutaneous CT-26 colon cancer tumors were significantly suppressed by the treatment of electrospun PU-PTX fibers without any systemic exposure of PTX. (Fig 2.) Annexin V binding study and histology (TUNEL assay and H&E staining) assured the long term biological activity of the released PTX and the apoptosis of tumor tissues after the localization of PTX.