Enhanced production of oleanolic acid by elicitation in suspension cell culture of Oldenlandia diffusa

초록

Oleanolic acid is a triterpenoid compound that exists in Oldenlandia diffusa. Recently, oleanolic acid has been noted for antitumor effect. The maximum dry cell weight in suspension cell of Oldenlandia diffusa was 22.8 g/L after 6 days of culture. Signal transducers and abiotic elicitors were added to at day 6 to increase the production of oleanolic acid. The maximum production of oleanolic acid was 99.6 mg/L in cultures with 0.5 mM salicylic acid. It is 1.74 times higher than that of control.