Ground organic monolith particles of improved separation efficiency in HPLC

초록

Ground organic monolith particles were prepared and packed in a glass-lined stainless steel column under high pressure and the separation performance was examined with a test mix composed of polar and nonpolar aromatic organic solutes. Methacrylic acid (functional monomrer), ethylene glycol dimethacryalte (EDMA, crosslinker), AIBN (initiator), and polyethylene glycol (PEG, porogen) were dissolved in toluene and heated at 60 oC for 24 h. The product was briefly smashed and washed with a series of solvents with stirring and dried. Choice of molecular weight of PEG was very critical to optimize separation performance. The number of theoretical plates of the column (1.0 X 300 mm) packed with this phase were over 10,000. Cheap production of ground organic monolith particles and their use as low cost separation media have been demonstrated in this study.