Isolation and Partial Characterization of Cryptic Polyene Gene Cluster in Pseudonorcardia autotrophica

초록

Using the polyene cytochrome P450 hydroxylase (CYP)-specific PCR screening with several actinomycetes genomic DNAs, Pseudonorcardia autotrophica strain was identified to contain a unique polyene-specific CYP gene. The complete sequencing of the cloned DNA revealed a total of seven complete and two incomplete open reading frames (ORFs), highly homologous but unique to previously-known polyene biosynthetic genes. These results suggest that the polyene-specific screening approach should be an efficient way of isolating potentially-valuable cryptic polyene biosynthetic gene cluster from various rare actinomycetes.