Chronological expression and interaction of INGAP and clusterin during pancreas regeneration

초록

In adult pancreas, new tissues can be developed by the process of neogenesis after tissue injury in order to maintain normal pancreatic function. During the pancreatic neogenesis, consecutive changes in molecular and morphological profile have been reported in the experimental models. We previously reported that clusterin, a glycoprotein, play a pivotal role in pancreatic neogenesis, while other investigators introduced other protein INGAP ( Islet Neogenesis Association Protein ) as a molecule having similar function to clusterin. The present study, thus, was aimed to reveal the interaction of the two neogenesis-associated proteins, clusterin and INGAP in the tissues of the various model of pancreatic neogenesis. Expression pattern of the clusterin and INGAP was monitored in the neogenic pancreas in each neogenic step of the rats with sub-total pancreatectomy (Px), the mice with pancreatic duct ligation, and the hamsters with cellophane wrapping. Expression of clusterin and INGAP was examined by immunocytochemistry, western blot analysis and RT-PCR, while interaction of the two proteins was determined by the method of coimmunoprecipitation.