Isolation of Three New Flavobacteriaceae Strains, Their Genome Characteristics, and Proposal of the Name Aurantibacter yeongjongensis gen. nov., sp. nov

초록

Three Gram-stain-negative, non-motile, orange-colored bacterium, designated IMCC2192T, IMCC2184, and IMCC2644 were isolated from a marine solar saltern. Cellular growth occurred at 25-30℃, pH 6.0-7.5, and with 1-4% (w/v) NaCl. The DNA G+C content of the strains were 35.9-36.0 mol%. The major respiratory quinone was menaquionone-6 (MK-6) and predominant cellular fatty acids were iso-C15:1 G, iso-C15:0 and iso-C17:0 3OH. Major polar lipids were aminolipids, phospholipids, phosphatidylethanolamine. Phylogenetic analysis based on 16S rRNA gene sequence indicated that the strains belonged to the family Flavobacteriaceae and formed a distinct lineage that was independent of closely related genera: Winogradskyella (95.1-92.8%), Flavirhabdus (94.2-94.3%), Bizionia (92.8-95.1%), and Corallibacter (93.9-94.0%). Average nucleotide identity (ANI) values (>98%) based on whole genome sequence data (IMCC2192T and IMCC2644) and DNA-DNA relatedness (>90%) based on hybridization (IMCC2192T and IMCC2184) indicated that the strains belong to the same genomics species. The genome size of strains IMCC2192T and IMCC2644 were 2.9 Mb and 2.8 Mb, respectively, and shared general genomic properties of the Flavobacteriaceae. In this regard, the strains are considered to represent a novel genus, for which Aurantibacter yeongjongensis gen. nov., sp. nov is proposed. The type strain is IMCC2192T (=KACC18774T). [Supported by a grant from the Marine Biotechnology Program (PJT200620) funded by the MOF, Korea]