Derivation of Insulin-producing Cells from Periosteum-derived Progenitor Cells in Vitro

초록

The major obstacle in cell therapy of type Ⅰ diabetes is the limited source of insulin-producing cells. Several research groups have studied various cell sources to generate insulin-producing cells that can be used for transplantation. Recently, periosteum-derived progenitor cells (PDPCs) which have similar characteristics of mesenchymal stem cells (MSCs) are easily isolated and have high growth potency. In this study, PDPCs are induced to trans-differentiate into insulin-producing cells (IPCs). For the differentiation into IPCs, PDPCs were cultured in high glucose Dulbecco's Modified Eagle Medium (DMEM) with different supplements under three-step condition for 18 days. IPCs derived from PDPCs showed increasing gene expression for insulin, glucagon, nestin, transcription factor PDX-1, Ngn3, Isl-1 Beta2/NeuroD and Pax4. The identity of the IPCs derived from PDPCs was illustrated by analyses of morphology and immunostaining. These results demonstrate that the PDPCs can differentiate into pancreatic-like cells as well as mesodermal cells. Therefore, we can conclude that PDPCs would help fulfill the urgent need of ample supply of IPCs for cell therapy in type Ⅰ diabetes.