Pelagibacterium sediminicola sp. nov., Isolated from Tidal Flat Sediment

초록

A Gram-stain-negative, aerobic, cream-coloured, non-gliding, motile with a single polar flagellum and rod-shaped bacterium, designated IMCC34151T, was isolated from tidal flat sediment of the Yellow sea, Republic of Korea. Phylogenetic analysis based 16S rRNA gene sequences indicated that strain IMCC34151T was affiliated with the genus Pelagibacterium in the family Hyphomicrobiaceae and shared 94.7-96.8 % sequence similarities with Pelagibacterium species. Whole genome sequencing of strain IMCC34151T revealed genome size of 3.2 Mbp and the G+C content of 62.6 mol%. The ranges of average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain IMCC34151T and other Pelagibacterium species were 75.4-74.0 % and 20.6-19.8 %, respectively. The strain contained summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), C19:0 cyclo ω8c and C16:0 as the major fatty acids and ubiquinone-10 (UQ-10) as the major respiratory quinone. The polar lipids detected in the strain were phosphatidylglycerol, diphosphatidylglycerol, two unidentified glycolipids and twelve unidentified lipids. On the basis of phylogenetic and phenotypic characteristics, strain IMCC34151T is considered to represent a novel species of the genus Pelagibacterium, for which the name Pelagibacterium sediminicola (type strain IMCC34151T = KACC 19595T = NBRC 113420T) sp. nov., is proposed. [Supported by a grant from the Collaborative Genome Program of the KIMST funded by the MOF (No. 20180430), Korea.]