Determination of Single and Competitive Isotherms of Phenol and o-Cresol in RP-HPLC

초록

There is a considerable industrial interest in both the scale-up and the optimization of chromatographic operations used in the purification of fine chemicals and biomolecules. One of the major factors affecting the adsorption operation is the adsorption isotherm. Reversed-phase high performance liquid chromatography(RP-HPLC) was used to measure the adsorption isotherm of phenol and o-cresol. From the experimental results, the retention times were decreased with increasing sample sizes, and the front of the peak was very stiff, so Langmuir adsorption isotherm was applied. Early-elution component, phenol, in mixture of the two components eltues faster than that in a pure component. Pulsed-injection method(PIM) was used to determine the two parameters of the adsorption isotherm. The resulting parameters, a and b, were used to calculate the elution profiles of phenol and o-cresol in pure and mixed state. The agreement between the experimental data and calculated elution profiles was fairly good in a mixture as well as a pure component.