Cultivation and Draft Genome Sequence of Marine Verrucomicrobial Strain IMCC8625

초록

The phylum Verrucomicrobia is widely distributed in marine environments, but only a limited number of verrucomicrobial isolates have been cultured from marine ecosystems. In this study, draft genome sequence and polyphasic taxonomy are reported for a verrucomicrobial strain IMCC8625 that was isolated from surface seawater of the East Sea by dilution-to-extinction cultivation. The 16S rRNA gene phylogeny revealed that strain IMCC8625 belong to the Verrucomicrobia subdivision 4 and is closely related to the genus Coraliomargarita akajimensis OSM45221T (95.4%, similarity) of the family Puniceicoccaceae Predominant fatty acids were revealed to be C18:1ω9c (23.0%), iSO-C14:0 (19.1%) and C14:0 (10.2%). The genome sequence of IMCC8625 was obtained using llumina sequencing. The 2.84 Mb genome was assembled into 69 contigs, with N50 of 93 kb. The ONA G+C content was 54.3 mol%. The genome of strain IMCC8625 has been found to possess 2,621 protein coding genes, including a gene for a protein FtsZ, a cytoskeletal protein involved in cell division. Additionally we found the presence of many ABC transpollers for proline/glycine betaine and amino acids.