Marine-derived bioink development for 3D printing

초록

3D printing (3DP) technology is the rapid and automized solid-form fabrication method with high reproducibility. Nevertheless, its development has not been expanded with natural polymer-based structural bioscaffolds with reliable mechanical integrity and high printing resolution. In this study, a new 3DP ink was engineered by mechanically durable and biocompatible sea anemone-derived silk-like protein (aneroin). The other marine biopolymers, such as hyaluronic acid (HA) and mussel adhesive protein (MAP), were also applied with aneroin for improved printability, mechanical properties, and biocompatibilities. Since this aneroin-based 3DP ink was able to be solidified by photo-crosslinking in a few second, spaces in printed 3D constructs were maintained without collapse. The multi-layer lattice, complex human ear and vascular graft were successfully bioprinted with high controllability and structural integrity.