Eleven Novel Species in the Genus Flavobacterium, Isolated from Nakdong River in Korea

초록

Eleven Gram-negative, non-motile, catalase-positive, rod-shaped bacterial strains, designated strains IMCC34515T, IMCC34518T, IMCC34673T, IMCC34774T, IMCC34775T, IMCC34776T, IMCC34777T, IMCC34779T, IMCC36791T,IMCC36792T, and IMCC36793T, were isolated from Nakdong River in Korea. Phylogenetic analyses based on 16S rRNA gene sequences indicated that all eleven strains are affiliated with the genus Flavobacterium. The 16S rRNA gene sequence similarities between the 11 strains and type strains of the genus were < 98.33%. Optimal growth of the 11 novel strains occurred on R2A at 20?25°C, at pH 7.0?8.0 and without NaCl. Major fatty acid constituents of these strains were iso-C15:0 and summed feature 3 (comprised C16:1 ω6c and/or C16:1 ω7c). Respiratory quinone detected in the strains was menaquinone-8 (MK-8). The draft genome sequences of the strains showed a rage of 3.4?5.8 Mb and average G + C contents of 33.41?37.32%. The genomes also showed ANI values of < 97.49% sequence similarities to each other and any currently described Flavobacterium species. This suggested that the 11 strains each represent different novel species of the genus Flavobacterium, therefore, we propose the establishment of 11 novel Flavobacterium species.