한국산 Rana 속 2종간의 유전적 차이 분석

초록

An analysis of the partial sequence of the mitochondrial cytochrome b gene was performed to investigate the intra- and interspecific differentiation among 2 species of the genus Rana(R.nigromaculata, R.plancyi). The sequence of the 315-base pairs, which cover approximately 28% of the cytochrome b gene, was determined by PCR-directed silver sequencing method. The sequence of Xenopus laevis(Roe et al.,1985) was utilized for outgroup comparision. Levels of mtDNA sequence differences were ranged from 0.3-3.2% within species, 3.8 - 6.4% between 2 Rana species and 24-28% between 2 species and Xenopus laevis. Transition is more frequent than transversion at synonymous site. Transitions were dominated at the third position of the codon. In amino acid analysis, number of amino acid differences were 14 amino acids between Rana species and X.laevis. Genetic distance was calculated by Tamura-Nei(1993).