식물세포배양에 의한 생물학적 변환에서의 cyclodextrin의 이용

초록

Addition of cyclodextrin in the biotransformation of digitoxin into digoxin by Digitalis lanata cell suspension cultures enhanced the conversion yield. Presence of cyclodextrin also supported good stability of the intermediate product for long time. Among several kinds of cyclodextrins, b-cyclodextrin showed the best results. It was found that the optimum form of cyclodextrin utilization was the external addition of inclusion complexes between digitoxin and b-cyclodextrin at 1:2 molar ratio. With the optimized conditions, addition of b-cyclodextrin in the biotranformation enhanced the production of digoxin 1.55 fold. In this case, not only digitoxin comsumptio was increased, but also the production of by-product was reduced.