Effects of Hydrolysates on Albumin-Erythropoietin Production in Recombinant CHO Cells

초록

In recombinant Chinese hamster ovary (rCHO) cell cultures, a particular attention has been paid to the design of an optimized medium in order to obtain high cell densities and enhanced productivity of recombinant proteins. For the achievement of these objectives, addition of various adding nutrient supplement was attempted and many results showed that the addition of various hydrolysates has been effective to enhance productivity. In this study, we added 3 g/L of yeast extract and phytopetone at day 3 to enhance the cell growth and production of albumin-erythropoietin(alb-EPO) supported maximum in rCHO cells. When we added the hydrolysates, it was found that yeast extract supported maximum cell density as well as alb-EPO concentration. Improvement of cell growth and alb-EPO production was 1.4-and 1.5-fold respectively. Lactate production, which is related with glucose consumption, was 2.4-fold lower than that of control culture without hydrolysates. These results suggested that the cultivation of rCHO cells with the addition of yeast extract was suitable for the improvement of cell growth and alb-EPO production.