Efficacy evaluation of Dandropanax morbifera extracts on indomethacin induced gastric mucosal damage in rats

초록

Dandropanax morbifera Lev. is belongs to araliaceae and can be found along the southern coast of South Korea and islands around. It has been shown anti-oxidation function as well as inhibition of liver damage. But its efficacy does not evaluated and confirmed on scientific methods such as in vivo approach with animal models. In the present study we have investigated the efficacy of Dandropanax morbifera extracts on its anti-ulcer effect using indomethacin induced gastric mucosal damage in rats. We divided the animals into 5 groups as follows: (1) vehicle control, (2) 25 mg/kg indomethacin, (3) 25 mg/kg indomethacin + 1.7ml/kg Dandropanax morbifera extracts, (4) 25 mg/kg indomethacin + 8.3ml/kg Dandropanax morbifera extracts, (5) 25 mg/kg indomethacin + 100mg/kg lansoprazole. In groups 2, 3, 4 and 5, indomethacin was given at 30 minutes after Dandropanax morbifera extracts administration. 25 mg/kg indomethacin was given to all rats (n=10) by oral gavage in 3% sodium bicarbonate. Seven hours after, the entire rats were sacrificed and confirmed gastrointestinal erosions and ulcers. Dandropanax morbifera extracts treatment before administration of indomethacin resulted in a decrease score in gastric damage areas in the entire group treated (P<0.05). It could be confirmed H&E staining with gut mucosal damage as well. And also, iNOS expression was decreased in Dandropanax morbifera extracts treatment group which is expressed very much in G2 group as an inflammation control. Our data suggests that Dandropanax morbifera extracts functions the protective effect on indomethacin-induced gastric mucosal damage.