Isolation of benzene tolerant bacteria and production of organic solvent stable enzymes from Bacillus species

초록

In the present study, the organic solvent stable protease and lipase producing bacterial strains were isolated from soil by benzene enrichment media. The positive strains were identified as Bacillus sp. PAP08 (Protease) and Bacillus subtilis LAP04 (lipase) by 16S rRNA sequencing method. The high level of protease and lipase was produced at 48 h of incubation. In addition, the enzyme stability was checked in different concentrations of benzene (1, 5, 25 and 50%). The result showed that the protease was completely stable and the activity was stimulated in all the tested concentration of benzene. The lipase activity was completely stable and enhanced up to 25% benzene. However, the enzyme activity was inhibited by 18% at 50% benzene. These organic solvent stable enzymes from Bacillus species may be useful for industrial applications in the presence of non-aqueous media. © 2019, World Research Association. All rights reserved.

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